Medical School Histology Basics - Introduction to Microscopy

The course provides an introduction to microscopy and the operation of a simple light microscope, of the type found in histology units and teaching laboratories. It outlines different methods used for preparing and staining tissue sections for microscopy, and explains how different stains can be used to identify particular cells, pathogens and anatomical structures.

Learning outcomes

After studying this course, you should be able to:

  • identify and understand the principle components of a light microscope
  • understand in outline, the principles of optics that apply to light microscopes
  • set up and use a light microscope
  • understand why different staining procedures are used on tissue sections
  • understand the principles of immunohistochemistry.

Course Curriculum

1. Microscopy
Microscopy 01:30:00
Using a light microscope 01:30:00
2. Additional systems on light microscopes
Phase contrast 01:30:00
Filters 01:00:00
Grids and graticules 01:00:00
3. Techniques
Histochemical techniques 01:30:00
4. Immunohistochemistry
Immunohistochemistry (IHC) 01:30:00
5. Interpreting the appearance of sections
Interpreting the appearance of sections 01:30:00
6. Fluorescence microscopes
Fluorescence microscopes 01:30:00
Conclusion – Microscopy 01:30:00
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